Comparison of Serum Biochemical and Mineral Profiles of Cattle Reared Under Island and Coastal Ecosystem

The present study was conducted in the Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, OUAT, BBSR in collaboration with ICAR-Central Island Agricultural Research Institute, Portblair (Andaman and Nicobar Island). Crossbred cattle with the history of anoestrus and repeat breeding were considered for the present investigation. The basic difference in serum biochemical and mineral profiles of cattle reared under two quite different ecosystems namely Island and coastal ecosystem was estimated which might be due to the variability in rainfall, ambient temperature, day light length and humidity. The incidence of different infertility conditions in both the ecosystems also might vary as per the environment. Serum samples were collected for estimations of biochemical parameters like total protein, glucose, cholesterol, Ca, P and Ca:P ratio. The overall incidence of infertility was recorded to be 47.62 % in island and 45.90 % in coastal ecosystem. No significant difference in serum levels of total protein, glucose, cholesterol, zinc, copper and manganese could be observed in between cattle reared in both ecosystems. It was concluded that the variations in humidity, ambient temperature, rain fall and day light length between Portblair, Andaman and coastal climate of Odisha, did not have much effect on reproduction of cattle

Tracing the genetic root of Trinket cattle: an endangered cattle population of a small isolated island of Nicobar group of islands

Trinket cattle are the inhabitant of a small island called Trinket, which is one of the picturesque islands of Andaman. This herd is thought to be of Danish leftover during their dynasty in Nicobar archipelago. When the island was abandoned by foreign invaders, indigenes utilized the animals for the purpose of meat. As a result, the cattle became semi-feral in nature. After the Great Sumatra earthquake and tsunami of Indian Ocean in 2004, Trinket island was left abandoned by indigenes and the cattle became totally feral in nature. To trace the genetic root of the cattle, this study has been undertaken based on the sequence information of the mitochondrial D-loop and cytochrome b gene. The genomic DNA was extracted from the blood samples of the Trinket cattle and was used for amplification of mitochondrial markers, and the sequence information was generated by Sanger sequencing. The analysis of sequence information revealed that the Trinket cattle belongs to Bos indicus (I) haplotype, sub-haplotype I2. The presence of I2 sub-haplotype in Trinket cattle may be due to the expansion of this I2 haplotype towards Southeast Asian countries. This is a novel input for the formulation of breeding strategy towards conservation of eco-friendly sustainable livestock in the isolated island ecosystem.

Variability of the EG95 antigen-coding gene of Echinococcus granulosus in animal and human origin: implications for vaccine development

In the present study, the genetic variability of the EG95 protein-coding gene in several animal and human isolates of Echinococcus granulosus was investigated. A total of 24 isolates collected from cattle, buffalo, sheep, goat, dog and man were amplified by Eg95-coding gene-specific primers. From the generated sequence information, a conceptual amino acid sequence was deduced. Phylogenetically, the Eg95 coding gene belongs to the Eg95-1/Eg95-2/Eg95-3/Eg95-4 cluster. Further confirmation on the maximum composite likelihood analysis revealed that the overall transition/transversion bias was 2.913. This finding indicated thatthere is bias towards transitional and transversional substitution. Using artificial neural networks, a B-cell epitope was predicted on primary sequence information. Stretches of amino acid residues varied between animal and human isolates when hydrophobicity was considered. Flexibility also varied between larval and adult stages of the organism. This observation is important to develop vaccines. However, cytotoxic T-lymphocyte epitopes on primary sequence data remained constant in all isolates. In this study, agretope identification started with hydrophobic amino acids. Amino acids with the same physico-chemical properties were present in the middle. The conformational propensity of the Eg95-coding gene of 156 amino acid residues had α-turns and β-turns, and α-amphipathic regions up to 129, 138–156 and 151–155 residues, respectively. The results indicated potential T-cell antigenic sites. The overall Tajima’s D value was negative (−2.404165), indicative of negative selection pressure.

Phytoplankton diversity in the Parangipettai coastal waters, southeast coast of India.

An investigation was carried out during June 2005 to May 2007 on hydrography, composition and community structure of phytoplankton including chlorophyll a in Parangipettai coastal waters (southeast coast of India). Air and surface water temperatures varied from 25.5 to 31.2 °C and from 25 to 29.3°C, respectively. Salinity values varied from 5 to 33.1‰ and the pH ranged between 7.2 and 8.3. Dissolved oxygen content varied from 3.1 to 7.9 mg l-1 while the light extinction coefficient values (LEC) ranged between 1.8 and 11.0 cm. The range of inorganic nutrients viz., nitrate, nitrite, phosphate and silicate were: 6.5 - 27.0 µM; 1.0 - 8.9 µM; 0.1 - 3.0 µM and 15.0 - 140 µM, respectively. The range of chlorophyll a was: 2.0 - 7.5 µg l-1. Presently, 117 phytoplankton species representing different classes viz: Bacillariophyceae (66); Dinophyceae (22); Cyanophyceae (19); Chlorophyceae (7) and Chrysophyceae (3) were recorded. The phytoplankton cell abundance varied from 0.14 to 132 cells ml-1, with peak diversity (3.52 bits ind.-1) during summer season. The maximum abundance was found during summer season coinciding with the stable hydrographical conditions. Canonical Correspondence Analysis was applied in this study for discriminating environmental factors effecting on phytoplankton community at species level.

Copepod population in Vellar estuary, Parangipettai coast in relation to environmental conditions.

Distribution and abundance of copepods were studied in relation to environmental conditions at two different ecosystems viz: Neritic (Bay of Bengal) and estuarine (Vellar estuary) of Parangipettai coast from September, 1998 to August, 2000. Over the study period, total 85 species of copepods were reported. Among these, the calanoid copepods constituted the major component with 63.52% followed by cyclopoids (29.41%) and harpacticoids (7.05%). The copepods population density was found to be high (2, 53, 000 org l-1) in estuarine water, while the species diversity was higher (5.47) in neritic water. The observed spatio-temporal variations in the population density and species diversity of copepods were more related to the environmental state of respective study area.

Studies on phytoplankton characteristics in Ayyampattinam coast, India.

Physico-chemical variables in the marine environment are subjected to wide spatio-temporal variations. The various physico-chemical parameters viz: temperature, salinity, pH, dissolved oxygen and nutrients of the environment are the factors which mainly influence the production and successful propagation of planktonic life in the coastal biotopes. The ranges of values of surface water temperature (0°C), salinity (‰), pH and dissolved oxygen (ml l-1) were: 25.5 - 33.4; 23 - 35; 7.8 - 8.2; 3.6 - 5.2, respectively. The values (Cg l-1) of nutrients were: nitrate 3.21 - 6.34, nitrite 0.74 - 0.896, phosphate 0.22 and 1.16, silicate 24.85 - 61.92 and ammonia 0.05 - 0.32. The recorded values of primary productivity (mgcm-3hr-1) ranged between 16 – 116 and the chlorophyll “a” varied from 3.74 – 8.52. A total number of 51 species of phytoplankton representing different classes viz: Bacillariophyceae (40); Dinophyceae (8); Chlorophyceae (1) and Cyanophyceae (2) was recorded. Among the four classes, Bacillariophyceae appeared to be the dominant group in respect of total species and cell numbers. The population density of phytoplankton was high during summer season and quite low during monsoon season.

Seasonal variations of plankton diversity in the Kaduviyar estuary,Nagapattinam, southeast coast of India.

The results of an investigation carried out during October 2005 to September 2006 on hydrography, composition and community structure of phytoplankton and zooplankton including chlorophyll ‘a’ (Chl-a) content and primary productivity (PP) at the Kaduviyar estuary (Southeast coast of India) are reported. Air and surface water temperatures (oC) varied from 23 to 29 and from 24 to 31 respectively while the light extinction coefficient values (LEC) ranged between 0.15 and 0.59. Salinity values (‰) varied from 4 to 34 and the pH ranged between 7.8 and 8.3. Variation in dissolved oxygen content was from 3.06 to 5.63 mg l-1. The ranges of inorganic nutrients (µM) viz., nitrate, nitrite, phosphate, silicate and ammonia were: 0.36-12.65; 0.06-1.86; 0.06-4.57; 17.96-235.06 and 0.001-0.75 respectively. The ranges of Chlorophyll ‘a’ (mg m-3) and the primary productivity (mgCm-3hr-1) values were: 3.4-12.8 and 55-119 respectively. Presently, 85 phytoplankton species representing different classes viz: Bacillariophyceae (58); Dinophyceae (16); Cyanophyceae (7); Chlorophyceae (3) and Chrysophyceae (1) were recorded. The phytoplankton density varied from 14,135 to 74,697 cells l-1, with peak diversity (3.46 bits ind.-1) during premonsoon season. The maximum density was found during summer season coinciding with the stable hydrographical conditions. Totally 92 species of zooplankton besides 18 larvae were recorded and the copepods formed the dominant group. The occurrence of most of the zooplankton species showed a distinct seasonal pattern, which was closely associated with the species-specific environmental conditions. That way the environmental factors exert major influence on the species composition, abundance and diversity of zooplankton. The zooplankton density (org. l-1) ranged between 4342 and 14,002 and between 4867 and 15,816 at stations 1 and 2 respectively. Kaduviyar estuarine waters were very rich in zooplankton diversity (bits ind.-1) viz: 5.29 at station 2 which was slightly higher than 5.27 at Station 1. Higher values of zooplankton density and species diversity were found during premonsoon and summer seasons and which showed positive correlation with salinity. The seasonal distribution and abundance of plankton are discussed in relation to hydrographical parameters.

Biochemical composition of wild copepods, Acartia spinicauda and Oithona similisfrom Parangipettai coastal waters in relation to environmental parameters.

Percentage composition of protein, lipid, carbohydrate and amino acids of copepods, Acartia spinicauda and Oithona similis collected from Parangipettai coastal waters were estimated following standard methodologies. Of the principal biochemical constituents, protein formed the major component followed by lipid and carbohydrate. Biochemical composition analysis of wild copepods indicated their nutritional rank. The ranges of protein, lipid and carbohydrate (%) contents (of A. spinicauda and O. similis) were 67.33-75.45; 12.42-16.58; 6.69-7.98 (Stn 1); 68.10-74.62; 12.65-17.81; 4.41-7.34 (Stn 2); 68.65-74.93; 14.55-17.69; 4.01-7.90 (Stn 3) and 59.53-69.61; 10.76-17.68; 3.43-6.59 (Stn 1); 62.39-67.09; 10.26-15.65; 3.78-5.85 (Stn 2) and 59.57-67.60; 9.89-15.44; 3.71-5.72 (Stn 3) respectively. Totally 16 amino acids were observed in these wild copepods, with threonine, glutamic acid, alanine, aspartic acid, serine, valine and methionine as the dominant ones. The minimum and maximum values of atmospheric and surface water temperatures (°C), salinity (‰), pH and dissolved oxygen (ml l-1) were 28.5-35; 28.7-34.2; 14.5-35.7; 7.4-8.6 and 3.0-7.2 respectively. The ranges (µM) of nitrate, nitrite, phosphate and silicate were 7.9-52.9; 0.6-9.6; 0.5-7.5 and 8.2-140.5 respectively. The results supported the view that protein may function as a metabolic reserve in copepods and that the availability of a constant supply of food may render large amount of lipid storage unnecessary in tropical copepods.

Phytoplankton diversity in Pichavaram mangrove waters from south-east coast of India.

The results of an investigation carried out during September 2002 to August 2003 on hydrography, composition and community structure of phytoplankton including chlorophyll ‘a’ (Chl-a) content and primary productivity (PP) of the Pichavaram mangrove (South-east coast of India) are reported. Atmospheric and surface water temperatures varied from 30.0 to 34.8oC and from 29.7 to 34.2oC respectively while the light extinction coefficient values (LEC) (K) ranged between 3.2 and 14.9. Salinity values varied from 9.6 to 35.4‰ and the pH ranged between 7.2 and 8.6. Variation in dissolved oxygen content was from 3.2 to 6.5 ml l-1. The ranges of inorganic nutrients viz., nitrate, nitrite, phosphate and silicate were: 7-36.23, 0.31-5.46, 0.28-3.70 and 12.26-56.64 μM respectively. Chlorophyll ‘a’ content ranged between 0.20 and 105.60 ;g l-1 and the ranges of gross and net primary productivities (PP) were: 16.54-826.8 and 11.52-610.2 mg C m-3 hr -1respectively. Presently a total of 94 species of phytoplankton were identified. Among these, the diatoms formed predominant group. Population density of phytoplankton varied from 400 to 3,21,000 cells-1. While the peak diversity (5.23 bits / ind.) of the phytoplankton was observed during summer season, the maximum population density was found during summer season coinciding with the stable hydrographical conditions.

Seasonal variations in physico-chemical characteristics of Pichavaram mangroves, southeast coast of India.

All the physico-chemical parameters such as temperature, salinity pH, dissolved oxygen and nutrients like nitrate, nitrite, inorganic phosphate and reactive silicate were studied in Pichavaram mangroves, southeast coast of India, for a period of two years (April 2000-March 2002). Air and surface water temperatures varied from 27 degrees C to 38 degrees C and from 26 degrees C to 37 degrees C. Salinity varied from 3.0 per thousand to 33.0 per thousand and the pH ranged between 7.2 and 8.2. Variation in dissolved oxygen content was from 2.4 to 5.0 ml l(-1). Concentrations of nutrients viz nitrates (9.50 to 32.12 microM), nitrites (1.07 to 5.99 microM), phosphates (0.73 to 2.36 microM) and reactive silicates (22.1 to 89.8 microM) also varied independently.

On the occurrence of a double parasitism (copepod and isopod) on the anchovy fish in India.

A double parasitism (isopod and copepod) on anchovy fish, Stolephorus commersonii Lacepede, 1803 was recorded from Parangipettai (India) coastal waters during June, 2004. This is the first report from this region and the infestation is discussed in relation to environmental parameters in this short communication.